- = a gold trophy

+ = a silver trophy

* = a bronze trophy

% = platinum

% Master Assassin - Unlock all trophies.

* The Birth of an Assassin - Be reborn as Ezio Auditore Da Firenze.

* Arrivederci Abstergo - Break out of Abstergo.

+ Welcome to the Animus 2.0 - Enter the Animus 2.0.

+ The Pain of Betrayal - Complete DNA Sequence 1.

+ Vengeance - Complete DNA Sequence 2.

+ Exit the Son - Complete DNA Sequence 3.

+ Bloody Sunday - Complete DNA Sequence 4.

+ Undertaker - Discover the Assassin's Tomb inside the catacombs under Santa Maria Novella.

+ The Conspirators - Complete DNA Sequence 5.

+ An Unexpected Journey - Complete DNA Sequence 6.

+ Bleeding Effect - Complete training and reenter the Animus.

+ The Merchant of Venice - Complete DNA Sequence 7.

+ The Impenetrable Palazzo - Complete DNA Sequence 8.

+ Masquerade - Complete DNA Sequence 9.

+ Bianca's Man - Complete DNA Sequence 10.

+ The Prophet - Complete DNA Sequence 11.

+ The Vault - Complete DNA Sequence 14.

- An Old Friend Returns - Escape the hideout.

* Myth Maker - Find the 8 statuettes in Monteriggioni.

* Vitruvian Man - Unlock all 20 pieces of Subject 16's video.

* Street Cleaner - Hide 5 dead bodies in a Bale of Hay.

* Fly Swatter - Kick a Guard while using the Flying Machine.

* Messer Sandman - Stun 4 guards at once by throwing sand in their face.

* Doctor - Perform an Air Assassination on a Poisoned NPC.

* No - Hitter - Kill 10 enemies while remaining in conflict without being hit.

* Kleptomaniac - Pickpocket 1000 Florins.

* Lightning Strike - Sprint for 100 meters.

* Sweeper - Sweep 5 guards at once by using a Long Weapon.

* Venetian Glatiator - Discover the Assassin's Tomb inside Santa Maria della Visitazione.

* I can see your house from here! - Discover the Assassin's Tomb inside the Torre Grossa.

* Hallowed be thy name - Discover the Assassin's Tomb inside the Basilica di San Marco.

* Prison Escape - Discover the Assassin's Tomb inside the Rocca di Ravaldino fortress.

* Choir Boy - Discover the Assassin's Tomb inside Santa Maria del Fiore ( The Duomo).

* Assassin For Hire - Complete your first assassination mission for Lorenzo II Magnifico.

* Macho Man - Defend a woman's honor.

* Steal Home - Win a race against thieves!

* Show your Colors - Wear the Auditore cape in each city.

* Handy Man - Upgrade a building in the Stronghold.

* I like the view - Synchronize 10 view points.

* High Dive - Perform a Leap of Faith from the Top of  Florence's Giotto's Campanile.

* Mailman - Intercept a Borgia Courrier.

* Tip of the Iceberg - Use your Eagle Vision to scan a Glyph in the environment.

* A Piece of the Puzzle - Unlock a piece of Subject 16's video.

* Art Connoisseur - Buy a painting from Florence and Venice.

* Podesta` of Monteriggioni - Reach 80% of your Stronghold's total value.

* Perfect Harmony - Tint your clothes with these colors : Wetland Ebony and Wetland Ivory.

* In Memory of Petruccio - Collect all the Feathers.

* Red Light Addict - Spend 5000 florins on Courtesans.

* Man of the People - Toss more than 300 florins on the ground.

* Victory lies in preparation - Get all Hidden Blades, Item pouches, and Armor Upgrades for Ezio.